Journal: Immunogenetics

Article Title: Two canine CD1a proteins are differentially expressed in skin

doi: 10.1007/s00251-008-0297-z

Figure Lengend Snippet: Flow cytometric analysis of expression canCD1 on tranfectant 293T cells. Cells are transfected with canCD1A6 ( a – b ) or with canCD1A8.2 gene transcripts ( c – d ). Staining was performed with anti-canine CD1 mAb CA13.9H11 and with anti-feline CD1a mAb, followed by GAM-FITC

Article Snippet: Anti-human CD1a (WM-35; IgG2b) was obtained from ImmunoTools.

Techniques: Expressing, Transfection, Staining

Journal: Immunogenetics

Article Title: Two canine CD1a proteins are differentially expressed in skin

doi: 10.1007/s00251-008-0297-z

Figure Lengend Snippet: a Molecular surface of human CD1a, canine CD1a6, and canine CD1a8. The α1 and α2 domains are depicted, and the opening to the binding groove is in the center of each molecule. Note that the bottom of the canCD1a6 binding groove appears electronegative due to the presence of Glu100; however, overall, the binding grove is hydrophobic. Furthermore, residue 100 is not highlighted in Fig. b, as it was not a residue contacting the sulfatide ligand in the human CD1a-sulfatide structure (1onq). Electrostatic potentials were calculated using APBS and PyMol; red is electronegative, and blue is electropositive (−30 to +30 kT/e). b Multiple-sequence alignment (CLUSTAL W 1.83) of canine CD1a isoforms 6 and 8 and human CD1a, colored by sequence identity ( black ) and structural features ( in color ). Residues contributing to the CD1a binding groove of human CD1a are highlighted in yellow . These residues were originally identified base on their interaction with the sulfatide ligand (PDB code 1onq). Darkgreen , A′ pole; green , terminal residue restricting the length of the A′ pocket; cyan , A′ roof; blue , residues at the opening to the CD1a groove (F′ portal). Residues colored in red or gray are similar. Residues that are highlighted but not colored in red are predicted not to dramatically alter the structural feature. Canine residues not highlighted according to their human counterparts are predicted to have an altered structural element

Article Snippet: Anti-human CD1a (WM-35; IgG2b) was obtained from ImmunoTools.

Techniques: Binding Assay, Residue, Sequencing

Journal: Immunogenetics

Article Title: Two canine CD1a proteins are differentially expressed in skin

doi: 10.1007/s00251-008-0297-z

Figure Lengend Snippet: a Alignment of the CD1a cytoplasmic tail sequences of different mammalian species. Tail sequences that were not confirmed by cDNA sequencing but only predicted from the genome are marked accordingly ( p ). b Alignment of cytoplasmic tail sequences of canCD1b, canCD1c, and canCD1d with their human orthologs. The tyrosine trafficking motif (YXXZ) is underlined

Article Snippet: Anti-human CD1a (WM-35; IgG2b) was obtained from ImmunoTools.

Techniques: Sequencing